Functional proteomics

Sinopse

As the emerging field of proteomics continues to expand at an extremely rapid rate, the relative quantification of proteins, targeted by their function, becomes its greatest challenge. Complex analytical strategies have been designed that allow comparative analysis of large proteomes, as well as in depth detection of the core proteome or the interaction network of a given protein of interest. Here expert researchers describe the latest protocols being developed to address the problems encountered in high-throughput proteomics projects. With emphasis on the factors governing the technical choices for given applications. As a part of the Methods in Molecular Biology. series, the chapters compile step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.